Monday, December 14, 2009

How does pore size aid in separation of molecules in electrophoresis?

and what does the sieving action means when it comes to these pores helping out in separation|||The smaller the pores the crisper the separation. The DNA has more trouble migrating through smaller pores. Everything moves more slowly, and fragments of the same length stay closer together.

Large fragments are sieved more (?) than smaller ones so they stay closer to the well, smaller ones move further and faster.

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